Stx3^{Gt(EUCE320f12)1.2Hmgu}
Gene trapped Allele Detail

Summary

• Symbol: Stx3^{Gt(EUCE320f12)1.2Hmgu}
• Name: syntaxin 3; gene trap EUCE320f12, derivative 1.2, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6355364
• Synonyms: Stx3^-
• Gene: Stx3 Location: Chr19:11752482-11796767 bp, - strand Genetic Position: Chr19, 8.52 cM
• Alliance: Stx3^{Gt(EUCE320f12)1.2Hmgu} page

Mutation origin

• Mutant Cell Line: EUCE320f12
• Germline Transmission: Earliest citation of germline transmission: J:275367
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion Vector: rsFlipROSAbgeo*
• Mutation details: A gene trap cassette flanked by inversely oriented pairs of the heterotypic FRT/F3 and lox511/loxP recombinase target sequences was inserted in intron 1 of the gene. Expression of FLP recombinase inverted the gene trap from its sense (mutagenic) orientation to antisense allowing expression of the gene (floxed allele). Subsequent Cre expression in the germline reinduced and fixed the mutation by the reinversion of gene trap to its sense orientation, generating a knockout allele. (J:275367)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stx3 Mutation: 16 strains or lines available

References

• Original: J:275367 Sanchez E, et al., Syntaxin 3, but not syntaxin 4, is required for mast cell-regulated exocytosis, where it plays a primary role mediating compound exocytosis. J Biol Chem. 2019 Mar 1;294(9):3012-3023
• All: 1 reference(s)