Stx4a^tm1.1Radac
Targeted Allele Detail

Summary

• Symbol: Stx4a^tm1.1Radac
• Name: syntaxin 4A (placental); targeted mutation 1.1, Roberto Adachi
• MGI ID: MGI:6355365
• Synonyms: Stx4^F
• Gene: Stx4a Location: Chr7:127439832-127450160 bp, + strand Genetic Position: Chr7, 69.77 cM, cytoband F4
• Alliance: Stx4a^tm1.1Radac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275367
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 1 (GRCm38; Chr7:127841278) and a second loxP site, followed by the phosphoglucokinase promoter-driven neomycin resistance gene (PGK-Neo) flanked by two FRT sites, was introduced in intron 4 (GRCm38; Chr7:127842997). The neomycin selection cassette was removed via Flp-mediated recombination, leaving exons 1-4 floxed. (J:275367)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stx4a Mutation: 22 strains or lines available

References

• Original: J:275367 Sanchez E, et al., Syntaxin 3, but not syntaxin 4, is required for mast cell-regulated exocytosis, where it plays a primary role mediating compound exocytosis. J Biol Chem. 2019 Mar 1;294(9):3012-3023
• All: 2 reference(s)