Arc^em1Pfw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Arc^em1Pfw
• Name: activity regulated cytoskeletal-associated protein; endonuclease-mediated mutation 1, Paul Worley
• MGI ID: MGI:6356322
• Synonyms: Arc^S260A/T278A
• Gene: Arc Location: Chr15:74540932-74544419 bp, - strand Genetic Position: Chr15, 34.25 cM
• Alliance: Arc^em1Pfw page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 mutagenesis was used to induce three mutations at different sites: two of these (CGA>AGC, TGT>AGC) caused the substitution of serine 260 and threonine 278 with alanine in the encoded protein (p.S260A, p.T278S). The third mutation is a synonymous substitution that removed the PAM site to prevent the sgRNA from binding to the edited allele. (J:276388)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arc Mutation: 29 strains or lines available

References

• Original: J:276388 Zhang W, et al., Arc Oligomerization Is Regulated by CaMKII Phosphorylation of the GAG Domain: An Essential Mechanism for Plasticity and Memory Formation. Mol Cell. 2019 May 23
• All: 1 reference(s)