Pacc1^em1.1Zzq
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pacc1^em1.1Zzq
• Name: proton activated chloride channel 1; endonuclease-mediated mutation 1.1, Zhaozhu Qiu
• MGI ID: MGI:6356325
• Synonyms: PAC^-
• Gene: Pacc1 Location: Chr1:191058109-191083111 bp, + strand Genetic Position: Chr1, 96.28 cM
• Alliance: Pacc1^em1.1Zzq page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis was used to insert two loxP sites, one 397 bp upstream of exon 2 and a second 309 bp dpwnstream of exon 2. Cre-mediated recombination deleted exon 2, resulting in a frameshift mutation before the TM1 domain and creating a null allele. qPCR analysis confirmed the absence of mRNA expression in homozygous mutant brains. (J:273611)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pacc1 Mutation: 25 strains or lines available

References

• Original: J:273611 Yang J, et al., PAC, an evolutionarily conserved membrane protein, is a proton-activated chloride channel. Science. 2019 Apr 26;364(6438):395-399
• All: 1 reference(s)