Qrich2^em1Xdz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Qrich2^em1Xdz
• Name: glutamine rich 2; endonuclease-mediated mutation 1, Xu-Dong Zhao
• MGI ID: MGI:6356346
• Gene: Qrich2 Location: Chr11:116332151-116357067 bp, - strand Genetic Position: Chr11, 81.3 cM
• Alliance: Qrich2^em1Xdz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced a frameshift mutation by deleting exon 1 and exon 2 of the gene. PCR, real-time PCR and western blot analysis confirmed the near absence of mRNA and protein expression in homozygous mutant testes. (J:270177)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Qrich2 Mutation: 54 strains or lines available

References

• Original: J:270177 Shen Y, et al., Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. Nat Commun. 2019 Jan 25;10(1):433
• All: 1 reference(s)