Sun5^em1Wli
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sun5^em1Wli
• Name: Sad1 and UNC84 domain containing 5; endonuclease-mediated mutation 1, Wei Li
• MGI ID: MGI:6356352
• Synonyms: Sun5^-13bp
• Gene: Sun5 Location: Chr2:153698108-153713004 bp, - strand Genetic Position: Chr2, 75.99 cM, cytoband H2
• Alliance: Sun5^em1Wli page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced a 13bp deletion of TGACCGCGGCCAG (reverse strand) in exon 10, causing a frameshift and premature stop codon. (J:247612)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Sun5^em1Wli/Sun5^em1Wli	involves: C57BL/6J * DBA/2
ht2	Sun5^em1Wli/Sun5^+	involves: C57BL/6J * DBA/2

Phenotypes:

Affected Systems	hm1		ht2
show or hide all annotated terms Sex:
cellular		√
teratozoospermia		√
abnormal sperm axoneme morphology		√
abnormal sperm connecting piece morphology		√
abnormal sperm head morphology		√
globozoospermia		√
absent sperm head		√
mortality/aging	N	N	N	N
mortality/aging	N	N	N	N
reproductive system		√		N
reproductive system phenotype		N		N
teratozoospermia		√
abnormal sperm axoneme morphology		√
abnormal sperm connecting piece morphology		√
abnormal sperm head morphology		√
globozoospermia		√
absent sperm head		√
abnormal spermiation		√
male infertility		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sun5 Mutation: 18 strains or lines available

References

• Original: J:247612 Shang Y, et al., Essential role for SUN5 in anchoring sperm head to the tail. Elife. 2017 Sep 25;6:e28199
• All: 3 reference(s)