Plekhn1^tm1.1Hkon
Targeted Allele Detail

Summary

• Symbol: Plekhn1^tm1.1Hkon
• Name: pleckstrin homology domain containing, family N member 1; targeted mutation 1.1, Hiroaki Konishi
• MGI ID: MGI:6356363
• Synonyms: CLPABP KO
• Gene: Plekhn1 Location: Chr4:156305913-156312999 bp, - strand Genetic Position: Chr4, 88.63 cM
• Alliance: Plekhn1^tm1.1Hkon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251603
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 3-8 and an adjacent frt-flanked neomycin selection cassette were deleted via Cre-mediated recombination of flanking loxP sites. RT-PCR analysis revealed the absence of mRNA expression in several tissues (including testes) and mouse embryonic fibroblasts (MEFs) obtained from homozygous mutant mice. Immunoblot analysis using a mouse specific antibody confirmed the absence of protein expression in mutant testes and MEFs. (J:251603)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plekhn1 Mutation: 55 strains or lines available

References

• Original: J:251603 Nishino T, et al., Antagonizing effect of CLPABP on the function of HuR as a regulator of ARE-containing leptin mRNA stability and the effect of its depletion on obesity in old male mouse. Biochim Biophys Acta. 2016 Nov;1861(11):1816-1827
• All: 1 reference(s)