Slc22a14^em1Fjw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc22a14^em1Fjw
• Name: solute carrier family 22 (organic cation transporter), member 14; endonuclease-mediated mutation 1, Wataru Fujii
• MGI ID: MGI:6356365
• Synonyms: Slc22a14^-, Slc22a14 KO
• Gene: Slc22a14 Location: Chr9:118998523-119019473 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Slc22a14^em1Fjw page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced a deletion of exons 7-9, as confirmed by genomic DNA sequencing and genome PCR. RT-PCR and Western blot analysis revealed that expression of mRNA and its product were prominently reduced in homozygous mutant testes. (J:277914)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc22a14 Mutation: 29 strains or lines available

References

• Original: J:277914 Maruyama SY, et al., A critical role of solute carrier 22a14 in sperm motility and male fertility in mice. Sci Rep. 2016 Nov 4;6:36468
• All: 2 reference(s)