Gpr62^em1Fjw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gpr62^em1Fjw
• Name: G protein-coupled receptor 62; endonuclease-mediated mutation 1, Wataru Fujii
• MGI ID: MGI:6356366
• Synonyms: Gpr62^-
• Gene: Gpr62 Location: Chr9:106341161-106343137 bp, - strand Genetic Position: Chr9, 57.56 cM
• Alliance: Gpr62^em1Fjw page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced a deletion of 334 base pairs, including the ATG codon. RT-PCR analysis showed that mRNA expression is significantly reduced in homozygous mutant testes. As the primers used for RT-PCR anneal downstream of the deleted region, it appears that mRNA is not transcribed or degraded rapidly. Although decreased protein expression could not be confirmed due to lack of appropriate antibody, authors believe that no protein is produced. (J:253644)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr62 Mutation: 5 strains or lines available

References

• Original: J:253644 Muroi T, et al., GPR62 constitutively activates cAMP signaling but is dispensable for male fertility in mice. Reproduction. 2017 Dec;154(6):755-764
• All: 1 reference(s)