Ppfia3^em1Pska
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ppfia3^em1Pska
• Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3; endonuclease-mediated mutation 1, Pascal S Kaeser
• MGI ID: MGI:6356372
• Synonyms: Liprin-alpha3^-
• Gene: Ppfia3 Location: Chr7:44988550-45016443 bp, - strand Genetic Position: Chr7, 29.26 cM
• Alliance: Ppfia3^em1Pska page

Mutation origin

• Strain of Origin: FVB/NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced an 8-bp deletion in exon 18. This resulted in a frame-shift mutation leading to nonsense-mediated decay. Western blot analysis of brain homogenates demonstrated the absence of protein expression in the hippocampi of homozygous mutant mice. (J:265782)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppfia3 Mutation: 47 strains or lines available

References

• Original: J:265782 Wong MY, et al., Liprin-alpha3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2234-2239
• All: 3 reference(s)