Spata46^tm1Itl
Targeted Allele Detail

Summary

• Symbol: Spata46^tm1Itl
• Name: spermatogenesis associated 46; targeted mutation 1, Ingenious Targeting Laboratory
• MGI ID: MGI:6356373
• Synonyms: Spata46^-
• Gene: Spata46 Location: Chr1:170136388-170139694 bp, + strand Genetic Position: Chr1, 76.84 cM
• Alliance: Spata46^tm1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236463
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2 of the gene were replaced with an FRT- and loxP flanked neomycin selection cassette. RT-PCR and Western blot analysis confirmed the absence of mRNA and protein expression, respectively, in the testes of homozygous mutant mice. (J:236463)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata46 Mutation: 9 strains or lines available

References

• Original: J:236463 Chen J, et al., Deficiency of SPATA46, a Novel Nuclear Membrane Protein, Causes Subfertility in Male Mice. Biol Reprod. 2016 Sep;95(3):58
• All: 1 reference(s)