Rbkstm1d(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Rbkstm1d(EUCOMM)Wtsi |
Name: |
ribokinase; targeted mutation 1d, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6356625 |
Synonyms: |
Rbksdelta2 |
Gene: |
Rbks Location: Chr5:31781783-31854971 bp, - strand Genetic Position: Chr5, 17.27 cM
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Alliance: |
Rbkstm1d(EUCOMM)Wtsi page
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IMPC: |
Rbks gene page |
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Mutant Cell Line: |
EPD0116_3_A10 |
Germline Transmission: |
Earliest citation of germline transmission:
J:236053
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 31830399 of Chromosome 5 upstream of the critical exon (exon 2) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 31831256. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and exon 2. RT-PCR analysis confirmed deletion of exon 2 at the RNA level.
(J:236053)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Rbks Mutation: |
72 strains or lines available
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Cell line EPD0116_3_A10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236053
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Original: |
J:236053 Liu Y, et al., Ribose Accelerates Gut Motility and Suppresses Mouse Body Weight Gaining. Int J Biol Sci. 2016;12(6):701-9 |
All: |
1 reference(s) |
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