Rtbdn^tm1.1Itl
Targeted Allele Detail

Summary

• Symbol: Rtbdn^tm1.1Itl
• Name: retbindin; targeted mutation 1.1, Ingenious Targeting Laboratory
• MGI ID: MGI:6356819
• Synonyms: Rtbdn^-
• Gene: Rtbdn Location: Chr8:85673620-85683232 bp, + strand Genetic Position: Chr8, 41.37 cM
• Alliance: Rtbdn^tm1.1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:257162
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.64kb eGFP cassette (eGFP-LoxP-FRT-Neo-FRT-LoxP) replaced 5.72Kb of genomic sequence, which flanks the Rtbdn start (ATG) codon and stop (TAG) codon. The neomycin selection cassette was removed via FLP-mediated recombination in the germline. Western blot analysis using an anti-Rtbdn antibody confirmed the absence of RTBDN protein expression in neural retina lysates from homozygous mutant mice. eEGFP expression was found exclusively in the neural retina and absent from all other tissues tested. Immunofluorescence revealed no co-localization of cone arrestin with native eGFP, suggesting that the Rtbdn promoter is not active in cones and it is specific to rod photoreceptors. (J:257162)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rtbdn Mutation: 9 strains or lines available

References

• Original: J:257162 Kelley RA, et al., Ablation of the riboflavin-binding protein retbindin reduces flavin levels and leads to progressive and dose-dependent degeneration of rods and cones. J Biol Chem. 2017 Dec 22;292(51):21023-21034
• All: 3 reference(s)