Clxn^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Clxn^tm1.1Osb
• Name: calaxin; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6356861
• Synonyms: Calaxin knockout, Efcab1^-, Efcab1^{tm1b(KOMP)Osb}
• Gene: Clxn Location: Chr16:14724315-14786925 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Clxn^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278278
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Targeting vector PG00105_W_3_A10 from the IKMC was used to generate the allele. An frt-lacZ-loxP-neo cassette-frt-loxP construct was inserted into intron 3 and a single loxP site was inserted into intron 4. Cre-mediated recombination of the loxP sites resulted in the removal of the neo cassette and exon 4 from the germline. No expression was detected in the sperm, trachea or ependyma of homozygous mutant mice at the mRNA or protein level. (J:278278)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clxn Mutation: 11 strains or lines available

References

• Original: J:278278 Sasaki K, et al., Calaxin is required for cilia-driven determination of vertebrate laterality. Commun Biol. 2019;2:226
• All: 1 reference(s)