Cdh10^{tm1.1(cre/ERT2)Jrs}
Targeted Allele Detail

Summary

• Symbol: Cdh10^{tm1.1(cre/ERT2)Jrs}
• Name: cadherin 10; targeted mutation 1.1, Joshua R Sanes
• MGI ID: MGI:6357709
• Synonyms: Cdh10^CreER
• Gene: Cdh10 Location: Chr15:18819033-19014322 bp, + strand Genetic Position: Chr15, 8.2 cM
• Alliance: Cdh10^{tm1.1(cre/ERT2)Jrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256032
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Cdh10^{tm1.1(cre/ERT2)Jrs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdh10 (MGI:107436)

• Mutation details: The targeting vector contained (from 5' to 3') a cDNA sequence encoding the CreER fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain) with N-terminal 6xMyc tag, followed by an FRT-flanked SV40-neo selection cassette. A 6XMyc-CreERT was inserted into the first coding exon (exon 2) of the cadherin-10 (Cdh10) gene by homologous recombination in mouse. Flp-mediated recombination removed the FRT-flanked neo cassette. Knockout of the cadherin-10 protein was confirmed biochemically and immunohistochemically. (J:268050)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdh10 (mouse) Summary of all recombinase alleles driven by Cdh10. Comparative matrix view of recombinase activities Alliance mouse Cdh10 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh10 Mutation: 45 strains or lines available

References

• Original: J:256032 Basu R, et al., Heterophilic Type II Cadherins Are Required for High-Magnitude Synaptic Potentiation in the Hippocampus. Neuron. 2017 Sep 27;96(1):160-176.e8
• All: 3 reference(s)