Hcrt^{tm1.1(flpo)Ksak}
Targeted Allele Detail

Summary

• Symbol: Hcrt^{tm1.1(flpo)Ksak}
• Name: hypocretin; targeted mutation 1.1, Kenji Sakimura
• MGI ID: MGI:6357834
• Synonyms: Hcrt^{tm1.1(flpo)Ahky}, Orexin-Flp
• Gene: Hcrt Location: Chr11:100652519-100653757 bp, - strand Genetic Position: Chr11, 63.6 cM
• Alliance: Hcrt^{tm1.1(flpo)Ksak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277436
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Hcrt^{tm1.1(flpo)Ksak} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hcrt (MGI:1202306)

• Mutation details: A targeting construct in which the MC1 promoter-driven diphtheria toxin gene, a 5.44 kb fragment at the 5' site, codon-optimized for mammalian expression Flp recombinase, including a nuclear localization signal cDNA fused to EGFP with the T2A peptide sequence, a Pgk-1 promoter-driven neomycin phosphotransferase gene (neo) flanked by two Dre recognition target (rox) sites was used for homologous recombination in ES cells to insert EGFP-2A-Flp just behind the translational initiation site of the hypocretin gene (Hcrt) in-frame. The neo cassette was removed in the ES cells by Dre recombination. This allele is a null allele confirmed by immunohistochemical studies. (J:277436)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hcrt (mouse) Summary of all recombinase alleles driven by Hcrt. Comparative matrix view of recombinase activities Alliance mouse Hcrt gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hcrt Mutation: 19 strains or lines available

References

• Original: J:277436 Chowdhury S, et al., Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice. Elife. 2019 Jun 4;8:e44927
• All: 4 reference(s)