Mecr^{tm1.1(fabI)Akast}
Targeted Allele Detail

Summary

• Symbol: Mecr^{tm1.1(fabI)Akast}
• Name: mitochondrial trans-2-enoyl-CoA reductase; targeted mutation 1.1, Alexander J Kastaniotis
• MGI ID: MGI:6357890
• Synonyms: Mecr^tm(fabI)
• Gene: Mecr Location: Chr4:131570781-131595097 bp, + strand Genetic Position: Chr4, 64.52 cM
• Alliance: Mecr^{tm1.1(fabI)Akast} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242847
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A sequence encoding the bacterial fabI ETR (2-enoyl-thioester reductase), a functional prokaryotic ortholog of Mecr, was introduced into exon 2 of the mouse Mecr gene. The construct was C-terminally tagged with a double HA epitope tag. The splicing acceptor sites of exon 2 and 54 nucleotides of the exon 2 itself were maintained in-frame with the fabI coding region. The Mecr mitochondrial targeting sequence (MTS) is encoded by exon 1. These alterations are predicted to yield a chimeric mRNA encoding HA-tagged fabI N-terminally extended with the Mecr MTS. The construct was followed by the strong transcriptional terminator BGH attached to an FRT-flanked selection cassette to exclude the possibility of producing intact Mecr. A Mecr^tm(fabl) mouse line devoid of the selection cassette was generated by deleting the FRT-flanked selection cassette by breeding with Tg(ACTFLPe)9205Dym/J mice. RT-PCR analysis revealed robust fabI mRNA transcript in E9.5 homozygous mutant embryos whereas fabI mRNA levels were ~25% in heterozygous embryos and essentially absent in wild-type embryos. Mecr mRNA expression was reduced to background levels and Western blot analysis confirmed the absence of Mecr protein expression in homozygous mutant embryos. A faint lipoylated Dlat signal was observed in protein extracts from homozygous embryos at E9.5, indicating a mild rescue of the enoyl-reductase deficiency. (J:242847)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecr Mutation: 17 strains or lines available

References

• Original: J:242847 Nair RR, et al., Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice. Hum Mol Genet. 2017 Jun 01;26(11):2104-2117
• All: 1 reference(s)