Elac2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Elac2^{tm1d(EUCOMM)Wtsi}
• Name: elaC ribonuclease Z 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6357904
• Synonyms: Elac2^-
• Gene: Elac2 Location: Chr11:64869864-64892895 bp, + strand Genetic Position: Chr11, 40.42 cM, cytoband B3
• Alliance: Elac2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Elac2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265839
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 64875939 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 64876709. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:265839)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Elac2 Mutation: 40 strains or lines available

References

• Original: J:265839 Siira SJ, et al., Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z. EMBO Rep. 2018 Oct;19(10)
• All: 2 reference(s)