Pcsk6^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcsk6^em1(IMPC)J
• Name: proprotein convertase subtilisin/kexin type 6; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6358003
• Gene: Pcsk6 Location: Chr7:65511884-65700134 bp, + strand Genetic Position: Chr7, 35.36 cM
• Alliance: Pcsk6^em1(IMPC)J page
• IMPC: Pcsk6 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GTTACAGTCAGAGTGATCCT and TCACAGAGAGTCCAAGACAA, which resulted in a 309 bp deletion beginning at Chromosome 7 position 65,928,994 bp and ending after 65,929,302 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000199980 (exon 5) and 232 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 209 and early truncation 7 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pcsk6 Mutation: 94 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)