Mettl14^tm1.1Czhao
Targeted Allele Detail

Summary

• Symbol: Mettl14^tm1.1Czhao
• Name: methyltransferase 14, N6-adenosine-methyltransferase subunit; targeted mutation 1.1, Jing Crystal Zhao
• MGI ID: MGI:6358166
• Synonyms: Mettl14^f
• Gene: Mettl14 Location: Chr3:123161944-123179639 bp, - strand Genetic Position: Chr3, 53.96 cM
• Alliance: Mettl14^tm1.1Czhao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258043
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting vector introduced into intron 1 a loxP site, an FRT site, splice acceptor, IRES, hygromycin resistance cassette, polyadenylation sequence, zeomycin resistance cassette, and FRT site. An additional loxP site was isnerted downstream of exon 2. Flp-mediated recombination removed the selection cassettes and left exon 2 floxed. (J:258043)

Expression

In Mice Carrying this Mutation:	89 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mettl14 Mutation: 36 strains or lines available

References

• Original: J:258043 Wang Y, et al., N(6)-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci. 2018 Feb;21(2):195-206
• All: 2 reference(s)