Serpina1^em#Chmu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Serpina1^em#Chmu
• Name: serine (or cysteine) peptidase inhibitor, clade A, member 1 cluster; endonuclease-mediated mutation, Christian Mueller
• MGI ID: MGI:6358591
• Gene: Serpina1 Location: unknown Genetic Position: Chr12, cytoband E-F

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intergenic deletion
• Serpina1^em#Chmu involves 4 genes/genome features (Serpina1b, Serpina1d, Serpina1a ...) View all
• Mutation details: CRISPR/Cas9 technology is used to disrupt five highly homologous gene paralogs through the targeting of exon 2 using a combination of four gRNAs. The population analyzed for phenotype contained individuals with alleles from the 3 lines (7A, 24B, 31C) and includes Serpina1^em1Chmu, Serpina1^em2Chmu, and Serpina1^em2Chmu. The pound symbol (#) is used when no line is specified or lines are pooled. Western blot and immunohistochemistry confirmed absence of the alpha1-antitrypsin protein in serum and liver tissue, respectively. (J:259615)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Serpina1 Mutation: 1 strain or line available

References

• Original: J:259615 Borel F, et al., Editing out five Serpina1 paralogs to create a mouse model of genetic emphysema. Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2788-2793
• All: 2 reference(s)