Orai2^tm1.2Itl
Targeted Allele Detail

Summary

• Symbol: Orai2^tm1.2Itl
• Name: ORAI calcium release-activated calcium modulator 2; targeted mutation 1.2, inGenious Targeting Laboratory
• MGI ID: MGI:6358680
• Synonyms: Orai2^L1F1
• Gene: Orai2 Location: Chr5:136176313-136199567 bp, - strand Genetic Position: Chr5, 75.84 cM
• Alliance: Orai2^tm1.2Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278536
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The exon starting at position 136,150,952 on chromosome 5 (exon 3 in Orai2-001 transcript and exon 5 in the Orai2-003 transcripit) was flanked by loxP sites. An FRT-flanked YFP-reporter (En2 splice acceptor, 2A self-cleavage protein, YFP coding sequence)-STOP-neomycin resistance cassette was inserted upstream of the floxed region. Flp-mediated recombination removed the YFP reporter and neo cassette and cre-mediated recombination deleted exon 3/exon5. qPCR confirmed absence of transcripts in peritoneal mast cells. (J:278536)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orai2 Mutation: 14 strains or lines available

References

• Original: J:278536 Tsvilovskyy V, et al., Deletion of Orai2 augments endogenous CRAC currents and degranulation in mast cells leading to enhanced anaphylaxis. Cell Calcium. 2018 May;71:24-33
• All: 1 reference(s)