Zc3h12c^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zc3h12c^{tm2c(EUCOMM)Hmgu}
• Name: zinc finger CCCH type containing 12C; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6359731
• Synonyms: Regnase-3^fl
• Gene: Zc3h12c Location: Chr9:52022644-52079872 bp, - strand Genetic Position: Chr9, 28.62 cM
• Alliance: Zc3h12c^{tm2c(EUCOMM)Hmgu} page
• IMPC: Zc3h12c gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277714
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 52031228 of Chromosome 9 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 52032143. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:277714)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zc3h12c Mutation: 36 strains or lines available

References

• Original: J:277714 von Gamm M, et al., Immune homeostasis and regulation of the interferon pathway require myeloid-derived Regnase-3. J Exp Med. 2019 Jul 1;216(7):1700-1723
• All: 1 reference(s)