Pdx1^{tm1.1(dre)Gkg}
Targeted Allele Detail

Summary

• Symbol: Pdx1^{tm1.1(dre)Gkg}
• Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, George K Gittes
• MGI ID: MGI:6359751
• Synonyms: Pdx1-Dre
• Gene: Pdx1 Location: Chr5:147206907-147212658 bp, + strand Genetic Position: Chr5, 86.84 cM
• Alliance: Pdx1^{tm1.1(dre)Gkg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278248
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Pdx1^{tm1.1(dre)Gkg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdx1 (MGI:102851)

• Mutation details: The targeting construct was generated such that exon 2 of the mouse Pdx1 gene in a BAC clone RP23-59N19 was replaced with a modified exon 2 in which a 2A peptide sequence followed by the Dre sequence was inserted immediately before the stop codon of the Pdx1-coding sequence. The FRT-flanked neomycin selection cassette (neo) was placed at the beginning of the 3' untranslated sequence which was removed by crossing chimeric mice with Flpo mice. (J:278248)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdx1 (mouse) Summary of all recombinase alleles driven by Pdx1. Comparative matrix view of recombinase activities Alliance mouse Pdx1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdx1 Mutation: 35 strains or lines available

References

• Original: J:278248 Chen C, et al., Evidence of a developmental origin for beta-cell heterogeneity using a dual lineage-tracing technology. Development. 2019 Jun 27;146(13):dev164913
• All: 1 reference(s)