Cpgi5563^{tm1.1(NCC)Wcs}
Targeted Allele Detail

Summary

• Symbol: Cpgi5563^{tm1.1(NCC)Wcs}
• Name: CpG island 5563; targeted mutation 1.1, William C Skarnes
• MGI ID: MGI:6359756
• Gene: Cpgi5563 Location: Chr13:48698873-48700447 bp Genetic Position: Chr13, Syntenic
• Alliance: Cpgi5563^{tm1.1(NCC)Wcs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277622
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5' loxP site was inserted upstream of the CpG island (CGI) of interest and replaced 72 bp of endogenous DNA (mm9, chr13:48,642,634-48,642,705). A rox-flanked STOP/selection cassette with a 3' FRT and loxP sites replaced 14 bp of genomic DNA (mm9, chr13:48,640,254-48,640,267). Treatment with a cell-permeant cre deleted the 2452 bp CGI (mm9, chr13:48,640,254-48,642,705). (J:277622)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cpgi5563 Mutation: 2 strains or lines available

References

• Original: J:277622 Koerner MV, et al., An Orphan CpG Island Drives Expression of a let-7 miRNA Precursor with an Important Role in Mouse Development. Epigenomes. 2019 Mar;3(1):7
• All: 1 reference(s)