Rab28^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rab28^{tm1d(EUCOMM)Wtsi}
• Name: RAB28, member RAS oncogene family; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6360451
• Synonyms: Rab28^-
• Gene: Rab28 Location: Chr5:41782319-41865500 bp, - strand Genetic Position: Chr5, 22.58 cM, cytoband B2
• Alliance: Rab28^{tm1d(EUCOMM)Wtsi} page
• IMPC: Rab28 gene page

Mutation origin

• Mutant Cell Line: EPD0688_3_D01
• Germline Transmission: Earliest citation of germline transmission: J:267522
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 41855482 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 41856303. The critical exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection exon, and exon 3. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:267522)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab28 Mutation: 18 strains or lines available

References

• Original: J:267522 Ying G, et al., The small GTPase RAB28 is required for phagocytosis of cone outer segments by the murine retinal pigmented epithelium. J Biol Chem. 2018 Nov 9;293(45):17546-17558
• All: 1 reference(s)