Vgll4^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Vgll4^{tm1c(EUCOMM)Hmgu}
• Name: vestigial like family member 4; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6360734
• Synonyms: Vgll4^fl
• Gene: Vgll4 Location: Chr6:114837589-114946955 bp, - strand Genetic Position: Chr6, 53.07 cM
• Alliance: Vgll4^{tm1c(EUCOMM)Hmgu} page
• IMPC: Vgll4 gene page

Mutation origin

• Mutant Cell Line: HEPD0680_7_D07
• Germline Transmission: Earliest citation of germline transmission: J:273437
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 114867338 of Chromosome 6 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 114868256. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:273437)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vgll4 Mutation: 24 strains or lines available

References

• Original: J:273437 Yu W, et al., VGLL4 plays a critical role in heart valve development and homeostasis. PLoS Genet. 2019 Feb;15(2):e1007977
• All: 3 reference(s)