Cog8^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cog8^em1(IMPC)J
• Name: component of oligomeric golgi complex 8; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6361033
• Gene: Cog8 Location: Chr8:107775341-107783369 bp, - strand Genetic Position: Chr8, 53.55 cM
• Alliance: Cog8^em1(IMPC)J page
• IMPC: Cog8 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATCATCAGCTTGAGGCCCCT and TACCAAGCACTTACTAGACA, which resulted in a 298 bp deletion beginning at Chromosome 8 position 107,054,004 bp and ending after 107,054,301 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001242994 (exon 2) and 90 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 132 and early truncation 2 amino acids later. There is a 4 bp insertion (GACG) at the deletion site. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cog8 Mutation: 36 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)