Lyar^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lyar^em1(IMPC)J
• Name: Ly1 antibody reactive clone; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6361103
• Gene: Lyar Location: Chr5:38377815-38391650 bp, + strand Genetic Position: Chr5, 20.29 cM
• Alliance: Lyar^em1(IMPC)J page
• IMPC: Lyar gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTAGGCTGACAGCATGCTAG and GGATCAAGATGGCATTTCCA, which resulted in a 2382 bp deletion beginning at Chromosome 5 position 38,225,848 bp and ending after 38,228,229 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000546144 through ENSMUSE00001264135 (exons 3-5) and 2075 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 41 and early truncation 59 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Lyar Mutation: 30 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)