Zfp74^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zfp74^em1(IMPC)J
• Name: zinc finger protein 74; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6361131
• Gene: Zfp74 Location: Chr7:29632086-29653579 bp, - strand Genetic Position: Chr7, 17.26 cM
• Alliance: Zfp74^em1(IMPC)J page
• IMPC: Zfp74 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACCCAGCCAGGTAAAGCGAG and AGGCAAGGCTGAAGTCTCCC, which resulted in a 921 bp deletion beginning at Chromosome 7 position 29,937,419 bp and ending after 29,938,339 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000965636 and ENSMUSE00000635760 (exons 3 and 4) and 698 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 6 and early truncation 19 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zfp74 Mutation: 101 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)