Wt1^{em1(cre/ERT2)Bzsh}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Wt1^{em1(cre/ERT2)Bzsh}
• Name: WT1 transcription factor; endonuclease-mediated mutation 1, Bin Zhou
• MGI ID: MGI:6361467
• Synonyms: Wt1-CrexER
• Gene: Wt1 Location: Chr2:104956874-105003959 bp, + strand Genetic Position: Chr2, 55.06 cM, cytoband E
• Alliance: Wt1^{em1(cre/ERT2)Bzsh} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Wt1^{em1(cre/ERT2)Bzsh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Wt1 (MGI:98968)

• Mutation details: The mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. A complementary DNA encoding Cre recombinase fused with 2 rox sequences flanking a mutant form of the ESR hormone-binding domain (ERT2) was inserted into the translational start site of the Wt1 gene and followed by a polyadenylation sequence. (J:278094)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wt1 (mouse) Summary of all recombinase alleles driven by Wt1. Comparative matrix view of recombinase activities Alliance mouse Wt1 gene page

Expression

In Mice Carrying this Mutation:

40 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wt1 Mutation: 34 strains or lines available

References

• Original: J:278094 Pu W, et al., Genetic Targeting of Organ-Specific Blood Vessels. Circ Res. 2018 Jun 22;123(1):86-99
• All: 1 reference(s)