Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik
Transgene Detail
|
Symbol: |
Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik |
Name: |
transgene insertion B22-1, M I Kotlikoff |
MGI ID: |
MGI:6361521 |
Synonyms: |
B22-LckBAC-Optoa1AR_IRES_lacZ, Lck-Opto alpha 1AR-IRES-lacZ |
Transgene: |
Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik Location: unknown
|
Alliance: |
Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik page
|
|
Strain of Origin: |
FVB/N X B6(Cg)-Tyrc-2J/J
|
|
Transgene Type: |
|
Transgenic (Reporter) |
Mutation: |
|
Insertion
|
|
|
Mutation details: The ~150 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP24-159E19 contains the entire lymphocyte protein tyrosine kinase locus as well as ~63 kbp of centromeric flanking sequences (including the complete Task3 and Hdac1 loci) and ~77 kbp of telomeric flanking sequences (including the complete Fam167b, Eif3i, Dcdc2b, Iqcc and Ccdc28b loci, as well as a portion of the Txlna locus). Using homologous recombination/BAC recombineering, a 6170 bp Optoalpha1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Lck gene. No other loci on the BAC were altered. The 13.4 kbp BAC vector contained a chloramphenicol selection cassette. The fusion protein is a Gt-coupled bovine rhodopsin (RHO) sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gq-coupled human alpha 1a-adrenergic receptor.
(J:314125)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
Original: |
J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858 |
All: |
1 reference(s) |
|