Scml1^tm1.2Atot
Targeted Allele Detail

Summary

• Symbol: Scml1^tm1.2Atot
• Name: Scm polycomb group protein like 1; targeted mutation 1.2, Attila Toth
• MGI ID: MGI:6362616
• Synonyms: Scml1^deletion
• Gene: Scml1 Location: ChrX:160598170-160608319 bp, - strand Genetic Position: ChrX, 74.15 cM
• Alliance: Scml1^tm1.2Atot page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278848
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT flanked lacZ-neo cassette and loxP site were inserted upstream of exon 7 and Rox flanked hyg-pgk cassette and loxP site were inserted downstream of exon 7 via homologous recombination. Flp and Dre mediated recombination removed the selection cassettes. Cre mediated recombination removed exon 7. Western blot analysis confirmed the absence of full length protein in testis extracts from hemizygous male mice (J:278848)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scml1 Mutation: 0 strains or lines available

References

• Original: J:278848 Papanikos F, et al., The enigmatic meiotic dense body and its newly discovered component, SCML1, are dispensable for fertility and gametogenesis in mice. Chromosoma. 2017 Jun;126(3):399-415
• All: 1 reference(s)