Lipa^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lipa^{tm1c(EUCOMM)Hmgu}
• Name: lysosomal acid lipase A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6364606
• Synonyms: Lal^fl
• Gene: Lipa Location: Chr19:34469718-34504874 bp, - strand Genetic Position: Chr19, 29.7 cM
• Alliance: Lipa^{tm1c(EUCOMM)Hmgu} page
• IMPC: Lipa gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270182
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34488106 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream exon 4 at position 34489566. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:270182)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lipa Mutation: 22 strains or lines available

References

• Original: J:270182 Leopold C, et al., Hepatocyte-specific lysosomal acid lipase deficiency protects mice from diet-induced obesity but promotes hepatic inflammation. Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Apr;1864(4):500-511
• All: 3 reference(s)