Sdc2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sdc2^{tm1c(KOMP)Wtsi}
• Name: syndecan 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6364846
• Synonyms: Sdc2^flox
• Gene: Sdc2 Location: Chr15:32920869-33034867 bp, + strand Genetic Position: Chr15, 13.74 cM
• Alliance: Sdc2^{tm1c(KOMP)Wtsi} page
• IMPC: Sdc2 gene page

Mutation origin

• Mutant Cell Line: EPD0107_2_G11
• Germline Transmission: Earliest citation of germline transmission: J:276769
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 33023250 of Chromosome 15 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 33024207. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:276769)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdc2 Mutation: 17 strains or lines available

References

• Original: J:276769 Corti F, et al., N-terminal syndecan-2 domain selectively enhances 6-O heparan sulfate chains sulfation and promotes VEGFA165-dependent neovascularization. Nat Commun. 2019 Apr 5;10(1):1562
• All: 2 reference(s)