Metrnl^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Metrnl^{tm1d(KOMP)Wtsi}
• Name: meteorin, glial cell differentiation regulator-like; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6367587
• Gene: Metrnl Location: Chr11:121592370-121607132 bp, + strand Genetic Position: Chr11, 86.89 cM
• Alliance: Metrnl^{tm1d(KOMP)Wtsi} page
• IMPC: Metrnl gene page

Mutation origin

• Mutant Cell Line: EPD0218_3_G08
• Germline Transmission: Earliest citation of germline transmission: J:268270
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 121598092 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 121599165. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268270)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Metrnl Mutation: 29 strains or lines available

References

• Original: J:268270 Ushach I, et al., Meteorin-like/Meteorin-beta Is a Novel Immunoregulatory Cytokine Associated with Inflammation. J Immunol. 2018 Dec 15;201(12):3669-3676
• All: 2 reference(s)