Mirc33^tm1.1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc33^tm1.1Wtsi
• Name: microRNA cluster 33, including Mir29a through Mir29b-1; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6367594
• Synonyms: miR-29a/b1^-
• Gene: Mirc33 Location: Chr6:31039595-31040028 bp Genetic Position: Chr6, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267719
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Mir29a and Mir29b1 in this microRNA cluster were replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by loxP and F3/FRT sites. Cre-mediated recombination excised the replacement cassette. (J:174268, J:267719)

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc33 Mutation: 0 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 3 reference(s)