Mirc29^tm1.1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc29^tm1.1Wtsi
• Name: microRNA cluster 29, including Mir29b-2 through Mir29c; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6367595
• Synonyms: miR-29b2/c^-
• Gene: Mirc29 Location: Chr1:194719348-194719942 bp Genetic Position: Chr1, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267719
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Mir29b2 and Mir29c in this microRNA cluster were replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by loxP and F3/FRT sites. Cre-mediated recombination excised the replacement cassette. (J:174268, J:267719)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Mirc29^tm1.1Wtsi/Mirc29^tm1.1Wtsi	C57BL/6N-Mirc29^tm1.1Wtsi
cx2	Mirc29^tm1.1Wtsi/Mirc29^tm1.1Wtsi Mirc33^tm1.1Wtsi/Mirc33^tm1.1Wtsi	C57BL/6N-Mirc29^tm1.1Wtsi Mirc33^tm1.1Wtsi

Phenotypes:

Affected Systems	hm1	cx2
show or hide all annotated terms
cardiovascular system	N	√
cardiovascular system phenotype	N
abnormal heart morphology		√
growth/size/body	√	√
decreased body weight	√	√
postnatal growth retardation		√
homeostasis/metabolism		√
increased fasting circulating glucose level		√
decreased circulating glucose level		√
decreased circulating insulin level		√
mortality/aging		√
premature death		√
renal/urinary system	√
ischuria	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc29 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)