Lamc1^tm2.1Ksek
Targeted Allele Detail

Summary

• Symbol: Lamc1^tm2.1Ksek
• Name: laminin, gamma 1; targeted mutation 2.1, Kiyotoshi Sekiguchi
• MGI ID: MGI:6367834
• Synonyms: Lamc1^cEQ
• Gene: Lamc1 Location: Chr1:153094668-153208532 bp, - strand Genetic Position: Chr1, 65.3 cM
• Alliance: Lamc1^tm2.1Ksek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279595
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Single point mutation
• Mutation details: A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 27 and a second loxP site and a copy of exon 28 containing a mutation that changes glutamic acid codon 1605 to a glutamine codon (p.E1605Q) were inserted into intron 28. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with the wild-type exon 28 (the last coding exon) floxed. Upon cre-mediated recombination, the floxed wild-type exon is deleted and the mutated exon 28 is expressed. (J:279595)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lamc1 Mutation: 143 strains or lines available

References

• Original: J:279595 Kiyozumi D, et al., Laminin gamma1 C-terminal Glu to Gln mutation induces early postimplantation lethality. Life Sci Alliance. 2018 Oct;1(5):e201800064
• All: 2 reference(s)