Tpm2^em1Eku
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tpm2^em1Eku
• Name: tropomyosin 2, beta; endonuclease-mediated mutation 1, Eri Kubo
• MGI ID: MGI:6368153
• Synonyms: Tpm2^-
• Gene: Tpm2 Location: Chr4:43514711-43523765 bp, - strand Genetic Position: Chr4, 23.05 cM, cytoband B1
• Alliance: Tpm2^em1Eku page

Mutation origin

• Strain of Origin: C57BL/6NCr

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Undefined
• Mutation details: CRISPR/Cas9 technology generated a global mutation, however information is not provided on the specific mutation except that the oligo-DNAs (5'-caccggagaatgccatcgaccgcg-3' and 5'-aaaccgcggtcgatggcattctcc-3') target all four isoforms. Real time PCR confirmed that levels of mRNA in the liver and heart are significantly lower in heterozygotes. (J:258198)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpm2 Mutation: 14 strains or lines available

References

• Original: J:258198 Shibata T, et al., Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev. 2018 Apr;171:24-30
• All: 1 reference(s)