Rrh^tm1Ght
Targeted Allele Detail

Summary

• Symbol: Rrh^tm1Ght
• Name: retinal pigment epithelium derived rhodopsin homolog; targeted mutation 1, Gabriel H Travis
• MGI ID: MGI:6368179
• Synonyms: Rrh^-
• Gene: Rrh Location: Chr3:129598057-129616236 bp, - strand Genetic Position: Chr3, 59.09 cM
• Alliance: Rrh^tm1Ght page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256777
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using homologous recombination in ES cells, a targeting vectir replaced a 4.3-kb fragment starting at the initiator methionine in exon 1 and a part of intron 1 of a mouse Rrh genomic fragment with a LacZ-coding region followed by a neomycin resistance cassette (PGK-Neo). Immunoblot analysis on retinal pigment epithelium (RPE) homogenates and immunohistochemistry of eyecup sections confirmed absence of protein in homozygous mice. It is a null allele. (J:256777)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rrh Mutation: 18 strains or lines available

References

• Original: J:256777 Cook JD, et al., Peropsin modulates transit of vitamin A from retina to retinal pigment epithelium. J Biol Chem. 2017 Dec 29;292(52):21407-21416
• All: 1 reference(s)