Gpat2^tm1.1Nkn
Targeted Allele Detail

Summary

• Symbol: Gpat2^tm1.1Nkn
• Name: glycerol-3-phosphate acyltransferase 2, mitochondrial; targeted mutation 1.1, Toru Nakano
• MGI ID: MGI:6368203
• Gene: Gpat2 Location: Chr2:127267119-127278012 bp, + strand Genetic Position: Chr2, 62.0 cM
• Alliance: Gpat2^tm1.1Nkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277128
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IKMC targeting vector was used to insert an FRT site, lacZ gene, loxP site, neomycin resistance gene, FRT site and loxP site upstream of exon 4 and a loxP site downstream of exon 6 via homologous recombination. Cre mediated recombination removed the neo cassette and exons 4 - 6. (J:277128)

Expression

In Mice Carrying this Mutation:	48 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpat2 Mutation: 53 strains or lines available

References

• Original: J:277128 Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
• All: 1 reference(s)