Cldn12^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Cldn12^{tm1.1(KOMP)Vlcg}
• Name: claudin 12; targeted mutation 1.1, Velocigene
• MGI ID: MGI:6368841
• Synonyms: claudin-12^lacZ, Cldn12^deltaneo
• Gene: Cldn12 Location: Chr5:5555109-5564953 bp, - strand Genetic Position: Chr5, 2.98 cM, cytoband A1
• Alliance: Cldn12^{tm1.1(KOMP)Vlcg} page
• IMPC: Cldn12 gene page

Mutation origin

• Mutant Cell Line: 13208A-A6
• Germline Transmission: Earliest citation of germline transmission: J:279934
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: Cre-mediated excision of the parental Cldn12^{tm1(KOMP)Vlcg} allele resulted in the removal of the loxP flanked neomycin selection cassette leaving behind the inserted lacZ reporter sequence. qRT-PCR of heart samples confirmed the successful insertion of the lacZ cassette in the open reading frame. (J:279934)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cldn12 Mutation: 21 strains or lines available

References

• Original: J:279934 Castro Dias M, et al., Claudin-12 is not required for blood-brain barrier tight junction function. Fluids Barriers CNS. 2019 Sep 12;16(1):30
• All: 1 reference(s)