Crisp2^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Crisp2^{tm1c(KOMP)Mbp}
• Name: cysteine-rich secretory protein 2; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:6369005
• Synonyms: Crisp2^flox
• Gene: Crisp2 Location: Chr17:41075625-41105037 bp, - strand Genetic Position: Chr17, 19.54 cM
• Alliance: Crisp2^{tm1c(KOMP)Mbp} page
• IMPC: Crisp2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:273472
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 41095297 of Chromosome 17 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 41096404. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:273472)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crisp2 Mutation: 12 strains or lines available

References

• Original: J:273472 Lim S, et al., CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility. Endocrinology. 2019 Apr 1;160(4):915-924
• All: 2 reference(s)