Bhlhe22^{tm1(flpo)Sros}
Targeted Allele Detail

Summary

• Symbol: Bhlhe22^{tm1(flpo)Sros}
• Name: basic helix-loop-helix family, member e22; targeted mutation 2, Sarah Ross
• MGI ID: MGI:6369826
• Synonyms: Bhlhb5::flpo
• Gene: Bhlhe22 Location: Chr3:18108489-18111678 bp, + strand Genetic Position: Chr3, 4.98 cM, cytoband A2
• Alliance: Bhlhe22^{tm1(flpo)Sros} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234119
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Bhlhe22^{tm1(flpo)Sros} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Bhlhe22 (MGI:1930001)

• Mutation details: The coding region of the gene was replaced with coding sequence for Flpo recombinase. An adjacent loxP-flanked neomycin selection cassette was removed via Cre-mediated recombination in the germline. (J:234119)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Bhlhe22 (mouse) Summary of all recombinase alleles driven by Bhlhe22. Alliance mouse Bhlhe22 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bhlhe22 Mutation: 12 strains or lines available

References

• Original: J:234119 Cai X, et al., Bhlhb5::flpo allele uncovers a requirement for Bhlhb5 for the development of the dorsal cochlear nucleus. Dev Biol. 2016 Jun 15;414(2):149-60
• All: 1 reference(s)