Cad^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cad^{tm1c(KOMP)Wtsi}
• Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6376189
• Synonyms: Cad^fl
• Gene: Cad Location: Chr5:31212124-31235823 bp, + strand Genetic Position: Chr5, 16.95 cM, cytoband B1
• Alliance: Cad^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0282_2_F10
• Germline Transmission: Earliest citation of germline transmission: J:260830
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31214943 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 31215660. The critical exon 3 is thus flanked by loxP sites. A conditional ready (floxed) allele is created by flp recombinase expression to remove the reporter and selection cassette, leaving exon 3 floxed. (J:260830)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cad Mutation: 75 strains or lines available

References

• Original: J:260830 Deng Y, et al., Adipocyte Xbp1s overexpression drives uridine production and reduces obesity. Mol Metab. 2018 May;11:1-17
• All: 1 reference(s)