Cd9^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cd9^{tm1c(EUCOMM)Hmgu}
• Name: CD9 antigen; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6376630
• Synonyms: Cd9^F
• Gene: Cd9 Location: Chr6:125437229-125471723 bp, - strand Genetic Position: Chr6, 59.32 cM
• Alliance: Cd9^{tm1c(EUCOMM)Hmgu} page
• IMPC: Cd9 gene page

Mutation origin

• Mutant Cell Line: HEPD0538_5_H11
• Germline Transmission: Earliest citation of germline transmission: J:280854
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 125441073 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 125441884. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280854)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd9 Mutation: 104 strains or lines available

References

• Original: J:280854 Wang VM, et al., CD9 identifies pancreatic cancer stem cells and modulates glutamine metabolism to fuel tumour growth. Nat Cell Biol. 2019 Nov 4
• All: 1 reference(s)