Actl6a^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actl6a^em1(IMPC)J
• Name: actin-like 6A; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6377435
• Gene: Actl6a Location: Chr3:32762695-32781122 bp, + strand Genetic Position: Chr3, 15.83 cM
• Alliance: Actl6a^em1(IMPC)J page
• IMPC: Actl6a gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TACTGTTCAGTGGATAACCC and ATATACTTCTTGAAGGAGAA, which resulted in a 345 bp deletion beginning at Chromosome 3 position 32,715,376 bp and ending after 32,715,720 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000171999 (exon 5) and 247 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 126 and early truncation 2 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Actl6a Mutation: 41 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)