Cyb5r3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyb5r3^{tm1c(KOMP)Wtsi}
• Name: cytochrome b5 reductase 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6378817
• Synonyms: Cyb5r3^fl
• Gene: Cyb5r3 Location: Chr15:83037695-83056793 bp, - strand Genetic Position: Chr15, 39.4 cM, cytoband E
• Alliance: Cyb5r3^{tm1c(KOMP)Wtsi} page
• IMPC: Cyb5r3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280543
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83046309 of Chromosome 15 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 83046870. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the selection cassette creating a conditional ready (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:280543)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyb5r3 Mutation: 19 strains or lines available

References

• Original: J:280543 Durgin BG, et al., Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation. JCI Insight. 2019 Oct 3;4(19)
• All: 5 reference(s)