P2ry12^{em1(icre/ERT2)Tda}
Endonuclease-mediated Allele Detail

Summary

• Symbol: P2ry12^{em1(icre/ERT2)Tda}
• Name: purinergic receptor P2Y, G-protein coupled 12; endonuclease-mediated mutation 1, Thomas D Arnold
• MGI ID: MGI:6379582
• Synonyms: P2ry12-CreER, P2Y12-CreER
• Gene: P2ry12 Location: Chr3:59123693-59170292 bp, - strand Genetic Position: Chr3, 29.01 cM
• Alliance: P2ry12^{em1(icre/ERT2)Tda} page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• P2ry12^{em1(icre/ERT2)Tda} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	P2ry12 (MGI:1918089)

• Mutation details: The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the P2ry12 locus on chromosome 3. The cas9-RNP complex and a 2A-iCreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the iCreER fusion gene with ~1.5 kbp homology arms) were injected into the pronucleus of C57BL/6N mouse embryos, followed by adoptive embryo transfer. Founder mice and progeny were screened by PCR, and proper genomic integration was verified by Southern blot and genomic sequencing. Founders were bred to C57BL/6J mice for germline transmission. (J:291145)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	P2ry12 (mouse) Summary of all recombinase alleles driven by P2ry12. Comparative matrix view of recombinase activities Alliance mouse P2ry12 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any P2ry12 Mutation: 41 strains or lines available

References

• Original: J:291145 McKinsey GL, et al., A new genetic strategy for targeting microglia in development and disease. Elife. 2020 Jun 23;9:e54590
• All: 5 reference(s)